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Transfected HEK293T cells were treated in triplicate for 4 hours with a serial dilution of IGF-1 LR3 which had been pre-incubated in conditioned media for 7 days

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Microscopy Retrobulbar application of L-NAME resulted in degeneration of ganglion cells and in nerve cell layer narrowing of the blood vessel lumen (using immunohistochemistry for Factor VIII)